Protein and Bio-medical Engineering

Transcriptome analysis & Gene Expression

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Transcriptome analysis and Gene Expression is the first and the important  topics to be discussed. While going in depth of the subject, it is necessary to understand Transcriptome as Key Players in Gene Expression. For that we must know the basics knowledge of how the central dogma works. This can be achieved by gaining proper knowledge about functioning of mRNA, tRNA and rRNA. Gene expression analysis experimentations can focus on a subset of relevant target genes. The location of gene and relative distances between genes on a chromosome can be determined through Sequence mapping. Even in the lack of the reference genome, transcriptome can be created using de novo transcriptome assembly method. Globally around more number of Universities and institutes are carrying research on gene expression and transcriptome analysis.